Using the LN34 Pan-Lyssavirus Real-Time RT-PCR Assay for Rabies Diagnosis and Rapid Genetic Typing from Formalin-Fixed Human Brain Tissue

Human rabies post mortem diagnostic samples are often preserved in formalin. While immunohistochemistry (IHC) has been routinely used for rabies antigen detection in formalin-fixed tissue, the formalin fixation process causes nucleic acid fragmentation that may affect PCR amplification. This study r...

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Detalhes bibliográficos
Publicado no:Viruses
Main Authors: Condori, Rene Edgar, Niezgoda, Michael, Lopez, Griselda, Matos, Carmen Acosta, Mateo, Elinna Diaz, Gigante, Crystal, Hartloge, Claire, Filpo, Altagracia Pereira, Haim, Joseph, Satheshkumar, Panayampalli Subbian, Petersen, Brett, Wallace, Ryan, Olson, Victoria, Li, Yu
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2020
Assuntos:
Case Report
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7019996/
https://ncbi.nlm.nih.gov/pubmed/31963651
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/v12010120
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