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Inference and effects of barcode multiplets in droplet-based single-cell assays

A widespread assumption for single-cell analyses specifies that one cell’s nucleic acids are predominantly captured by one oligonucleotide barcode. Here, we show that ~13–21% of cell barcodes from the 10x Chromium scATAC-seq assay may have been derived from a droplet with more than one oligonucleoti...

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Pubblicato in:Nat Commun
Autori principali: Lareau, Caleb A., Ma, Sai, Duarte, Fabiana M., Buenrostro, Jason D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7018801/
https://ncbi.nlm.nih.gov/pubmed/32054859
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-14667-5
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