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Toward Standards in Clinical Microbiota Studies: Comparison of Three DNA Extraction Methods and Two Bioinformatic Pipelines

When studying the microbiome using next-generation sequencing, the DNA extraction method, sequencing procedures, and bioinformatic processing are crucial to obtain reliable data. Method choice has been demonstrated to strongly affect the final biological interpretation. We assessed the performance o...

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Podrobná bibliografie
Vydáno v:mSystems
Hlavní autoři: Ducarmon, Q. R., Hornung, B. V. H., Geelen, A. R., Kuijper, E. J., Zwittink, R. D.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7018525/
https://ncbi.nlm.nih.gov/pubmed/32047058
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSystems.00547-19
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