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Toward Standards in Clinical Microbiota Studies: Comparison of Three DNA Extraction Methods and Two Bioinformatic Pipelines

When studying the microbiome using next-generation sequencing, the DNA extraction method, sequencing procedures, and bioinformatic processing are crucial to obtain reliable data. Method choice has been demonstrated to strongly affect the final biological interpretation. We assessed the performance o...

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Detalhes bibliográficos
Publicado no:mSystems
Main Authors: Ducarmon, Q. R., Hornung, B. V. H., Geelen, A. R., Kuijper, E. J., Zwittink, R. D.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7018525/
https://ncbi.nlm.nih.gov/pubmed/32047058
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSystems.00547-19
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