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Toward Standards in Clinical Microbiota Studies: Comparison of Three DNA Extraction Methods and Two Bioinformatic Pipelines

When studying the microbiome using next-generation sequencing, the DNA extraction method, sequencing procedures, and bioinformatic processing are crucial to obtain reliable data. Method choice has been demonstrated to strongly affect the final biological interpretation. We assessed the performance o...

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Bibliografiske detaljer
Udgivet i:mSystems
Main Authors: Ducarmon, Q. R., Hornung, B. V. H., Geelen, A. R., Kuijper, E. J., Zwittink, R. D.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7018525/
https://ncbi.nlm.nih.gov/pubmed/32047058
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSystems.00547-19
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