An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue

A fluorescence marker mOrange was inserted to the popular pLentiCrispr-V2 to create pLentiCrispr-V2-mOrange (V2mO) that contained both a puromycin selection and a fluorescent marker, making viral production and target transduction visible. Lentiviruses packaged with this plasmid and appropriate guid...

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Publicado en:PLoS One
Autores principales: Jin, Jiankang, Xu, Yan, Huo, Longfei, Ma, Lang, Scott, Ailing W., Pizzi, Melissa Pool, Li, Yuan, Wang, Ying, Yao, Xiaodan, Song, Shumei, Ajani, Jaffer A.
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7018052/
https://ncbi.nlm.nih.gov/pubmed/32053639
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0228910
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