BIG-TREE: Base-Edited Isogenic hPSC Line Generation Using a Transient Reporter for Editing Enrichment

Current CRISPR-targeted single-nucleotide modifications and subsequent isogenic cell line generation in human pluripotent stem cells (hPSCs) require the introduction of deleterious double-stranded DNA breaks followed by inefficient homology-directed repair (HDR). Here, we utilize Cas9 deaminase base...

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Publicado en:Stem Cell Reports
Main Authors: Brookhouser, Nicholas, Tekel, Stefan J., Standage-Beier, Kylie, Nguyen, Toan, Schwarz, Grace, Wang, Xiao, Brafman, David A.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7013208/
https://ncbi.nlm.nih.gov/pubmed/32004495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2019.12.013
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