Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging

Two-photon excitation microscopy is one of the key techniques used to observe three-dimensional (3-D) structures in biological samples. We utilized a visible-wavelength laser beam for two-photon excitation in a multifocus confocal scanning system to improve the spatial resolution and image contrast...

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Bibliographic Details
Published in:J Biomed Opt
Main Authors: Oketani, Ryosuke, Suda, Haruka, Uegaki, Kumiko, Kubo, Toshiki, Matsuda, Tomoki, Yamanaka, Masahito, Arai, Yoshiyuki, Smith, Nicholas I., Nagai, Takeharu, Fujita, Katsumasa
Format: Artigo
Language:Inglês
Published: Society of Photo-Optical Instrumentation Engineers 2019
Subjects:
Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7008499/
https://ncbi.nlm.nih.gov/pubmed/31691550
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.JBO.25.1.014502
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