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PSF engineering in multifocus microscopy for increased depth volumetric imaging

Imaging and localizing single molecules with high accuracy in a 3D volume is a challenging task. Here we combine multifocal microscopy, a recently developed volumetric imaging technique, with point spread function engineering to achieve an increased depth for single molecule imaging. Applications in...

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Detalhes bibliográficos
Publicado no:Biomed Opt Express
Main Authors: Hajj, Bassam, El Beheiry, Mohamed, Dahan, Maxime
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4866451/
https://ncbi.nlm.nih.gov/pubmed/27231584
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.7.000726
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