Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy

Reversibly switchable fluorescent proteins (RSFPs) serve as markers in advanced fluorescence imaging. Photoswitching from a non-fluorescent off-state to a fluorescent on-state involves trans-to-cis chromophore isomerization and proton transfer. Whereas excited-state events on the ps timescale have b...

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Publicado no:Nat Commun
Main Authors: Woodhouse, Joyce, Nass Kovacs, Gabriela, Coquelle, Nicolas, Uriarte, Lucas M., Adam, Virgile, Barends, Thomas R. M., Byrdin, Martin, de la Mora, Eugenio, Bruce Doak, R., Feliks, Mikolaj, Field, Martin, Fieschi, Franck, Guillon, Virginia, Jakobs, Stefan, Joti, Yasumasa, Macheboeuf, Pauline, Motomura, Koji, Nass, Karol, Owada, Shigeki, Roome, Christopher M., Ruckebusch, Cyril, Schirò, Giorgio, Shoeman, Robert L., Thepaut, Michel, Togashi, Tadashi, Tono, Kensuke, Yabashi, Makina, Cammarata, Marco, Foucar, Lutz, Bourgeois, Dominique, Sliwa, Michel, Colletier, Jacques-Philippe, Schlichting, Ilme, Weik, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7005145/
https://ncbi.nlm.nih.gov/pubmed/32029745
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-14537-0
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