A SYBR green I–based quantitative RT-PCR assay for bovine ephemeral fever virus and its utility for evaluating viral kinetics in cattle

We developed a SYBR green I–based reverse-transcription quantitative PCR (RT-qPCR) assay for bovine ephemeral fever virus (BEFV). Analytical sensitivity of the assay was ~ 100 times higher than conventional RT-PCR. The precision of the RT-qPCR established for RNA standards was high, with intra-assay...

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Detalhes bibliográficos
Publicado no:J Vet Diagn Invest
Main Authors: Gao, Shandian, Du, Junzheng, Tian, Zhancheng, Niu, Qingli, Huang, Dexuan, Wang, Jidong, Luo, Jianxun, Liu, Guangyuan, Yin, Hong
Formato: Artigo
Idioma:Inglês
Publicado em: SAGE Publications 2019
Assuntos:
Full Scientific Reports
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7003230/
https://ncbi.nlm.nih.gov/pubmed/31845623
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/1040638719895460
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