Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR

BACKGROUND: Bovine viral diarrhea virus (BVDV) is a worldwide pathogen in cattle and acts as a surrogate model for hepatitis C virus (HCV). One-step real-time fluorogenic quantitative reverse transcription polymerase chain reaction (RT-PCR) assay based on SYBR Green I dye has not been established fo...

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Detalhes bibliográficos
Main Authors: Zhang, Ni, Liu, Zhengwen, Han, Qunying, Qiu, Jianming, Chen, Jinghong, Zhang, Guoyu, Li, Zhu, Lou, Sai, Li, Na
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2011
Assuntos:
Methodology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3157457/
https://ncbi.nlm.nih.gov/pubmed/21798067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-8-374
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