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Droplet Digital PCR with EvaGreen Assay: Confirmational Analysis of Structural Variants
We describe a variation of droplet digital PCR (ddPCR) for DNA structural variant analysis, where expensive fluorescent probes are replaced by low-cost EvaGreen, a DNA binding dye, hence reducing cost without reducing sensitivity. ddPCR is a method that employs water-oil microfluidics and fluorescen...
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| Publicado no: | Curr Protoc Hum Genet |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7001142/ https://ncbi.nlm.nih.gov/pubmed/30040209 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cphg.58 |
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