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Use of green fluorescent protein to monitor fungal growth in biomass hydrolysate

A reporter gene encoding green fluorescent protein (GFP) was introduced into the ascomycete Coniochaeta ligniaria NRRL30616, and fluorescence of cultures was monitored as a measure of cell growth. Fluorescence in the GFP-expressing strain was measured during growth of cells in defined and complex me...

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Bibliographic Details
Published in:Biol Methods Protoc
Main Authors: Nichols, Nancy N, Quarterman, Joshua C, Frazer, Sarah E
Format: Artigo
Language:Inglês
Published: Oxford University Press 2018
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6994092/
https://ncbi.nlm.nih.gov/pubmed/32161794
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpx012
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