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Use of Green Fluorescent Protein To Monitor Cell Envelope Stress in Lactococcus lactis
A Lactococcus lactis reporter system suitable to detect cell envelope stress in high-throughput settings was developed by fusing the CesR-regulated promoter of llmg0169 to the gfp(uv) gene. A dot blot assay allowed fast detection of green fluorescent protein (GFP) fluorescence even at low production...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
2010
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2813010/ https://ncbi.nlm.nih.gov/pubmed/19948854 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02177-09 |
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