CD-tagging-MS2: detecting allelic expression of endogenous mRNAs and their protein products in single cells

Discriminating between the mRNA and protein outputs of each of the alleles of an endogenous gene in intact cells, is a difficult task. To examine endogenous transcripts originating from a specific allele, we applied Central Dogma tagging (CD-tagging), which is based on a tag insertion into an endoge...

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
में प्रकाशित:Biol Methods Protoc
मुख्य लेखकों: Sheinberger, Jonathan, Hochberg, Hodaya, Lavi, Erez, Kanter, Itamar, Avivi, Shira, Reinitz, Gita, Schwed, Avital, Aizler, Yuval, Varon, Eli, Kinor, Noa, Shav-Tal, Yaron
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: Oxford University Press 2017
विषय:
Methods Manuscript
ऑनलाइन पहुंच:https://ncbi.nlm.nih.gov/pmc/articles/PMC6994078/
https://ncbi.nlm.nih.gov/pubmed/32161787
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpx004
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