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Covalent modification of primers improves PCR amplification specificity and yield
We report a method for covalent modification of primers that enhances the specificity of PCR and increases the yield of specific amplification products at the end of PCR. The introduction of thermally stable covalent modifications, such as alkyl groups to the exocyclic amines of deoxyadenosine or cy...
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| Udgivet i: | Biol Methods Protoc |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6994073/ https://ncbi.nlm.nih.gov/pubmed/32161793 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpx011 |
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