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Covalent modification of primers improves PCR amplification specificity and yield
We report a method for covalent modification of primers that enhances the specificity of PCR and increases the yield of specific amplification products at the end of PCR. The introduction of thermally stable covalent modifications, such as alkyl groups to the exocyclic amines of deoxyadenosine or cy...
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| Publicado no: | Biol Methods Protoc |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6994073/ https://ncbi.nlm.nih.gov/pubmed/32161793 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpx011 |
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