Covalent modification of primers improves PCR amplification specificity and yield

We report a method for covalent modification of primers that enhances the specificity of PCR and increases the yield of specific amplification products at the end of PCR. The introduction of thermally stable covalent modifications, such as alkyl groups to the exocyclic amines of deoxyadenosine or cy...

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Bibliographic Details
Published in:Biol Methods Protoc
Main Authors: Schoenbrunner, Nancy J, Gupta, Amar P, Young, Karen K Y, Will, Stephen G
Format: Artigo
Language:Inglês
Published: Oxford University Press 2017
Subjects:
Methods Manuscript
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6994073/
https://ncbi.nlm.nih.gov/pubmed/32161793
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpx011
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