Učitavanje...
qPCR-based characterization of DNA fragmentation efficiency of Tn5 transposomes
Here, we describe an electrophoresis free assay for characterizing Tn5 transposomes fragmentation efficiency in a tagmentation reaction, in which double-stranded DNA is fragmented and tagged with adapter sequences. The assay uses plasmid DNA as a reference tagmentation substrate. Fragmentation effic...
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| Izdano u: | Biol Methods Protoc |
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| Glavni autori: | , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
2017
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6994069/ https://ncbi.nlm.nih.gov/pubmed/32161784 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpx001 |
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