Exome Sequencing from Nanogram Amounts of Starting DNA: Comparing Three Approaches

Hybridization-based target enrichment protocols require relatively large starting amounts of genomic DNA, which is not always available. Here, we tested three approaches to pre-capture library preparation starting from 10 ng of genomic DNA: (i and ii) whole-genome amplification of DNA samples with R...

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Detalhes bibliográficos
Principais autores: Rykalina, Vera N., Shadrin, Alexey A., Amstislavskiy, Vyacheslav S., Rogaev, Evgeny I., Lehrach, Hans, Borodina, Tatiana A.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4081514/
https://ncbi.nlm.nih.gov/pubmed/24992588
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0101154
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