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Improving the efficiency of gene insertion in a human artificial chromosome vector and its transfer in human-induced pluripotent stem cells

A human artificial chromosome (HAC) vector has potential to overcome the problems of stable gene expression associated with plasmid, transposon, and virus-based vectors, such as insertional mutagenesis, position effect, uncontrollable copy number, unstable gene expression, and DNA size limitation. T...

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Detalhes bibliográficos
Publicado no:Biol Methods Protoc
Main Authors: Hasegawa, Yoshinori, Ikeno, Masashi, Suzuki, Nobutaka, Nakayama, Manabu, Ohara, Osamu
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6994043/
https://ncbi.nlm.nih.gov/pubmed/32161806
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpy013
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