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Choice of library size normalization and statistical methods for differential gene expression analysis in balanced two-group comparisons for RNA-seq studies

BACKGROUND: High-throughput RNA sequencing (RNA-seq) has evolved as an important analytical tool in molecular biology. Although the utility and importance of this technique have grown, uncertainties regarding the proper analysis of RNA-seq data remain. Of primary concern, there is no consensus regar...

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Bibliografiske detaljer
Udgivet i:BMC Genomics
Main Authors: Li, Xiaohong, Cooper, Nigel G. F., O’Toole, Timothy E., Rouchka, Eric C.
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6986029/
https://ncbi.nlm.nih.gov/pubmed/31992223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-020-6502-7
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