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Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F
Intracellular pH (pH(i)) plays a crucial role in mammalian sperm physiology. However, it is a challenging task to acquire quantitative single sperm pH(i) images due to their small size and beating flagella. In this study, we established a robust pH(i) imaging system using the dual-emission ratiometr...
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| Publicado en: | Front Cell Dev Biol |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Frontiers Media S.A.
2020
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6978660/ https://ncbi.nlm.nih.gov/pubmed/32010689 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fcell.2019.00366 |
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