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Live imaging of intracellular pH in planarians using the ratiometric fluorescent dye SNARF-5F-AM

Physiological parameters such as resting potential and pH are increasingly recognized as important regulators of cell activity and tissue-level events in regeneration, development, and cancer. The availability of fluorescent reporter dyes has greatly increased the ability to track these properties i...

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Dades bibliogràfiques
Publicat a:Biol Methods Protoc
Autors principals: Beane, Wendy Scott, Adams, Dany Spencer, Morokuma, Junji, Levin, Michael
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6541873/
https://ncbi.nlm.nih.gov/pubmed/31206034
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpz005
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