載入...
Reverse-transcription quantitative PCR directly from cells without RNA extraction and without isothermal reverse-transcription: a ‘zero-step’ RT-qPCR protocol
We describe an ultra-rapid and sensitive method to quantify gene expression levels in cultured cells. The procedure is based on reverse-transcription quantitative PCR (RT-qPCR) directly from cells, without RNA extraction and without an isothermal reverse-transcription step. Human neurons (Lund human...
Na minha lista:
| 發表在: | Biol Methods Protoc |
|---|---|
| Main Authors: | , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
2017
|
| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6977950/ https://ncbi.nlm.nih.gov/pubmed/32002469 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpx008 |
| 標簽: |
添加標簽
沒有標簽, 成為第一個標記此記錄!
|