Reverse-transcription quantitative PCR directly from cells without RNA extraction and without isothermal reverse-transcription: a ‘zero-step’ RT-qPCR protocol

We describe an ultra-rapid and sensitive method to quantify gene expression levels in cultured cells. The procedure is based on reverse-transcription quantitative PCR (RT-qPCR) directly from cells, without RNA extraction and without an isothermal reverse-transcription step. Human neurons (Lund human...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:Biol Methods Protoc
Main Authors: Chovancova, Petra, Merk, Verena, Marx, Andreas, Leist, Marcel, Kranaster, Ramon
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2017
Assuntos:
Methods Manuscript
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6977950/
https://ncbi.nlm.nih.gov/pubmed/32002469
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpx008
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados