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Building better enzymes: Molecular basis of improved non‐natural nucleobase incorporation by an evolved DNA polymerase

Obtaining semisynthetic microorganisms that exploit the information density of “hachimoji” DNA requires access to engineered DNA polymerases. A KlenTaq variant has been reported that incorporates the “hachimoji” P:Z nucleobase pair with a similar efficiency to that seen for Watson–Crick nucleobase i...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Protein Sci
Main Authors: Ouaray, Zahra, Singh, Isha, Georgiadis, Millie M., Richards, Nigel G. J.
Format: Artigo
Jezik:Inglês
Izdano: John Wiley & Sons, Inc. 2019
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6954703/
https://ncbi.nlm.nih.gov/pubmed/31654473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3762
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