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Sequencing artifacts derived from a library preparation method using enzymatic fragmentation

DNA fragmentation is a fundamental step during library preparation in hybridization capture-based, short-read sequencing. Ultra-sonication has been used thus far to prepare DNA of an appropriate size, but this method is associated with a considerable loss of DNA sample. More recently, studies have e...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Tanaka, Norio, Takahara, Akihisa, Hagio, Taichi, Nishiko, Rika, Kanayama, Junko, Gotoh, Osamu, Mori, Seiichi
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6941819/
https://ncbi.nlm.nih.gov/pubmed/31899787
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0227427
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