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Use of GapmeRs for gene expression knockdowns in human primary resting CD4+ T cells

Human primary resting CD4+ T cells are difficult to transfect while preserving viability. The present study evaluated gymnotic delivery and RNase H1-dependent gene expression knockdown mediated by antisense oligonucleotides, called GapmeRs. Exposure of primary resting CD4+ T cells to GapmeRs did not...

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Bibliographische Detailangaben
Veröffentlicht in:J Immunol Methods
Hauptverfasser: Abewe, Hosiana, Deshmukh, Savitha, Mukim, Amey, Beliakova-Bethell, Nadejda
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2019
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6939142/
https://ncbi.nlm.nih.gov/pubmed/31629740
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2019.112674
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