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K-means Spectral Unmixing for Multi-channel Imaging and Image Analysis Platform at a Core Facility

There is an increasing need of multi-channel fluorescent imaging to simultaneously visualize different biological structures and the dynamic interactions between them. However, due to the overlapping emission spectra of many commonly used fluorophores, many times fluorescent images have bleed-throug...

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Pubblicato in:J Biomol Tech
Autori principali: Gao, Yurong, McRae, Tristan
Natura: Artigo
Lingua:Inglês
Pubblicazione: Association of Biomolecular Resource Facilities 2019
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6936903/
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