A Flow Cytometric Method to Quantify the Endosomal Escape of a Protein Toxin to the Cytosol of Target Cells

PURPOSE: The aim of this work was to develop a quantitative, flow cytometric method for tracking the endolysosomal escape of a fluorescently labelled saporin toxin. METHODS: Flow cytometric measurements of fluorescent pulse width and height were used to track the endocytic uptake into Daudi cells of...

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Vydáno v:Pharm Res
Hlavní autoři: Wensley, Harrison J., Johnston, David A., Smith, Wendy S., Holmes, Suzanne E., Flavell, Sopsamorn U., Flavell, David J.
Médium: Artigo
Jazyk:Inglês
Vydáno: Springer US 2019
Témata:
Research Paper
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6928089/
https://ncbi.nlm.nih.gov/pubmed/31873810
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11095-019-2725-1
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