Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae

A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ(5)‐3β‐hydroxysteroid dehydrogenase gene from Digitalis lanata...

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Bibliografiske detaljer
Udgivet i:Microbiologyopen
Main Authors: Rieck, Christoph, Geiger, Daniel, Munkert, Jennifer, Messerschmidt, Katrin, Petersen, Jan, Strasser, Juliane, Meitinger, Nadine, Kreis, Wolfgang
Format: Artigo
Sprog:Inglês
Udgivet: John Wiley and Sons Inc. 2019
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Original Articles
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6925150/
https://ncbi.nlm.nih.gov/pubmed/31436030
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/mbo3.925
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