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Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae
A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ(5)‐3β‐hydroxysteroid dehydrogenase gene from Digitalis lanata...
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| Publicado no: | Microbiologyopen |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
John Wiley and Sons Inc.
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6925150/ https://ncbi.nlm.nih.gov/pubmed/31436030 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/mbo3.925 |
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