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Image-based analysis of living mammalian cells using label-free 3D refractive index maps reveals new organelle dynamics and dry mass flux

Holo-tomographic microscopy (HTM) is a label-free microscopy method reporting the fine changes of a cell’s refractive indices (RIs) in three dimensions at high spatial and temporal resolution. By combining HTM with epifluorescence, we demonstrate that mammalian cellular organelles such as lipid drop...

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Detalhes bibliográficos
Publicado no:PLoS Biol
Main Authors: Sandoz, Patrick A., Tremblay, Christopher, van der Goot, F. Gisou, Frechin, Mathieu
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6922317/
https://ncbi.nlm.nih.gov/pubmed/31856161
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pbio.3000553
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