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PRIMEval: Optimization and screening of multiplex oligonucleotide assays

The development of multiplex polymerase chain reaction and microarray assays is challenging due to primer dimer formation, unspecific hybridization events, the generation of unspecific by-products, primer depletion, and thus lower amplification efficiencies. We have developed a software workflow wit...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Conzemius, Rick, Hendling, Michaela, Pabinger, Stephan, Barišić, Ivan
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6917790/
https://ncbi.nlm.nih.gov/pubmed/31848453
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-55883-4
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