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PRIMEval: Optimization and screening of multiplex oligonucleotide assays

The development of multiplex polymerase chain reaction and microarray assays is challenging due to primer dimer formation, unspecific hybridization events, the generation of unspecific by-products, primer depletion, and thus lower amplification efficiencies. We have developed a software workflow wit...

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Publicat a:Sci Rep
Autors principals: Conzemius, Rick, Hendling, Michaela, Pabinger, Stephan, Barišić, Ivan
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6917790/
https://ncbi.nlm.nih.gov/pubmed/31848453
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-55883-4
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