Fast and Reliable PCR Amplification from Aspergillus fumigatus Spore Suspension Without Traditional DNA Extraction

Aspergillus fumigatus is an opportunistic human pathogenic mold. DNA extraction from this fungus is usually performed by mechanical perturbation of cells, as it possesses a rigid and complex cell wall. While this is not problematic for single isolates, it can be time consuming for large numbers of s...

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Foilsithe in:Curr Protoc Microbiol
Main Authors: Fraczek, Marcin G., Zhao, Can, Dineen, Lauren, Lebedinec, Ressa, Bowyer, Paul, Bromley, Michael, Delneri, Daniela
Formáid: Artigo
Teanga:Inglês
Foilsithe: John Wiley and Sons Inc. 2019
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Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6916316/
https://ncbi.nlm.nih.gov/pubmed/31518062
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmc.89
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