Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes

While Tn-Seq is a powerful tool to determine genome-wide bacterial fitness in high-throughput, culturing transposon-mutant libraries in pools can mask community or other complex single-cell phenotypes. Droplet Tn-Seq (dTn-Seq) solves this problem by microfluidics facilitated encapsulation of individ...

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Publicat a:Nat Commun
Autors principals: Thibault, Derek, Jensen, Paul A., Wood, Stephen, Qabar, Christine, Clark, Stacie, Shainheit, Mara G., Isberg, Ralph R., van Opijnen, Tim
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2019
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6914776/
https://ncbi.nlm.nih.gov/pubmed/31844066
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-13719-9
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