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Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes
While Tn-Seq is a powerful tool to determine genome-wide bacterial fitness in high-throughput, culturing transposon-mutant libraries in pools can mask community or other complex single-cell phenotypes. Droplet Tn-Seq (dTn-Seq) solves this problem by microfluidics facilitated encapsulation of individ...
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| Izdano u: | Nat Commun |
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| Glavni autori: | , , , , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Nature Publishing Group UK
2019
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6914776/ https://ncbi.nlm.nih.gov/pubmed/31844066 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-13719-9 |
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