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Nutrient germination improves DNA recovery from industrial Bacillus subtilis endospores during qPCR enumeration assays

Growth-independent microbial enumeration methods such as quantitative PCR require the efficient extraction of genomic DNA from targeted cells. Bacillus endospores are popular inclusions in commercial products due to their hardiness and metabolic dormancy; however, this hardiness is known to render B...

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Vydáno v:Heliyon
Hlavní autoři: Gorsuch, John P., Woodruff, Peyton
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6911885/
https://ncbi.nlm.nih.gov/pubmed/31872115
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.heliyon.2019.e02917
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