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Fluorescence Energy Transfer Immunoassay Based on a Long-Lifetime Luminescent Metal–Ligand Complex

We describe an imunoassay based on fluorescence resonance energy transfer (FRET). The antigen was human serum albumin (HSA), which was labeled with a ruthenium-ligand complex, [Ru(bpy)(2)(phen-ITC)](2+). The antibody (IgG) to HSA was labeled with a nonfluorescent absorber, Reactive Blue 4. Associati...

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Bibliografiske detaljer
Udgivet i:Anal Biochem
Main Authors: Youn, Hee Ju, Terpetschnig, Ewald, Szmacinski, Henryk, Lakowicz, Joseph R.
Format: Artigo
Sprog:Inglês
Udgivet: 1995
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6907077/
https://ncbi.nlm.nih.gov/pubmed/8600827
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1006/abio.1995.9966
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