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Fluorescence Energy Transfer Immunoassay Based on a Long-Lifetime Luminescent Metal–Ligand Complex
We describe an imunoassay based on fluorescence resonance energy transfer (FRET). The antigen was human serum albumin (HSA), which was labeled with a ruthenium-ligand complex, [Ru(bpy)(2)(phen-ITC)](2+). The antibody (IgG) to HSA was labeled with a nonfluorescent absorber, Reactive Blue 4. Associati...
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| Publicado en: | Anal Biochem |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1995
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6907077/ https://ncbi.nlm.nih.gov/pubmed/8600827 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1006/abio.1995.9966 |
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