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Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein(1)
We describe a glucose sensor based on a mutant glucose/galactose binding protein (GGBP) and phase-modulation fluorometry. The GGBP from Escherichia coli was mutated to contain a single cysteine residue at position 26. When labeled with a sulfhydryl-reactive probe 2-(4′-iodoacetamidoanilino)naphthale...
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| Publié dans: | Anal Biochem |
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| Auteurs principaux: | , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1999
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6905191/ https://ncbi.nlm.nih.gov/pubmed/9918662 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1006/abio.1998.2974 |
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