Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein(1)

We describe a glucose sensor based on a mutant glucose/galactose binding protein (GGBP) and phase-modulation fluorometry. The GGBP from Escherichia coli was mutated to contain a single cysteine residue at position 26. When labeled with a sulfhydryl-reactive probe 2-(4′-iodoacetamidoanilino)naphthale...

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Bibliografske podrobnosti
izdano v:Anal Biochem
Main Authors: Tolosa, Leah, Gryczynski, Ignacy, Eichhorn, Lisa R., Dattelbaum, Jonathan D., Castellano, Felix N., Rao, Govind, Lakowicz, Joseph R.
Format: Artigo
Jezik:Inglês
Izdano: 1999
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6905191/
https://ncbi.nlm.nih.gov/pubmed/9918662
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1006/abio.1998.2974
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