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Generation of targeted homozygosity in the genome of human induced pluripotent stem cells

When loss of heterozygosity (LOH) is correlated with loss or gain of a disease phenotype, it is often necessary to identify which gene or genes are involved. Here, we developed a region-specific LOH-inducing system based on mitotic crossover in human induced pluripotent stem cells (hiPSCs). We first...

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Dettagli Bibliografici
Pubblicato in:PLoS One
Autori principali: Yoshimura, Yasuhide, Yamanishi, Ayako, Kamitani, Tomo, Kim, Jin-Soo, Takeda, Junji
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2019
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6894808/
https://ncbi.nlm.nih.gov/pubmed/31805151
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0225740
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