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The Methyltransferase Region of Vesicular Stomatitis Virus L Polymerase Is a Target Site for Functional Intramolecular Insertion

The L-protein of vesicular stomatitis virus (VSV) is a single-chain multi-domain RNA-dependent RNA polymerase. Previously reported attempts of intramolecular insertions of fluorescent proteins into the L-protein resulted in temperature-sensitive and highly attenuated polymerase activity. Here, we de...

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Publicat a:Viruses
Autors principals: Heilmann, Emmanuel, Kimpel, Janine, Geley, Stephan, Naschberger, Andreas, Urbiola, Carles, Nolden, Tobias, von Laer, Dorotheé, Wollmann, Guido
Format: Artigo
Idioma:Inglês
Publicat: MDPI 2019
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6893670/
https://ncbi.nlm.nih.gov/pubmed/31717818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/v11110989
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