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Robust blind spectral unmixing for fluorescence microscopy using unsupervised learning

Due to the overlapping emission spectra of fluorophores, fluorescence microscopy images often have bleed-through problems, leading to a false positive detection. This problem is almost unavoidable when the samples are labeled with three or more fluorophores, and the situation is complicated even fur...

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Dades bibliogràfiques
Publicat a:PLoS One
Autors principals: McRae, Tristan D., Oleksyn, David, Miller, Jim, Gao, Yu-Rong
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6886781/
https://ncbi.nlm.nih.gov/pubmed/31790435
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0225410
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