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Evolution of a Reverse Transcriptase to Map N(1)-Methyladenosine in Human mRNA

Chemical modifications on messenger RNA are increasingly recognized as a critical regulatory layer in the flow of genetic information, but quantitative tools to monitor RNA modifications in a whole-transcriptome and site-specific manner are lacking. Here we describe a versatile directed evolution pl...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Zhou, Huiqing, Rauch, Simone, Dai, Qing, Cui, Xiaolong, Zhang, Zijie, Nachtergaele, Sigrid, Sepich, Caraline, He, Chuan, Dickinson, Bryan C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6884687/
https://ncbi.nlm.nih.gov/pubmed/31548705
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-019-0550-4
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