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Poly(A) inclusive RNA isoform sequencing (PAIso−seq) reveals wide-spread non-adenosine residues within RNA poly(A) tails

Message RNA poly(A) tails are vital for their function and regulation. However, the full-length sequence of mRNA isoforms with their poly(A) tails remains undetermined. Here, we develop a method at single-cell level sensitivity that enables quantification of poly(A) tails along with the full-length...

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Vydáno v:Nat Commun
Hlavní autoři: Liu, Yusheng, Nie, Hu, Liu, Hongxiang, Lu, Falong
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6876564/
https://ncbi.nlm.nih.gov/pubmed/31757970
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-13228-9
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