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Effective CRISPR interference of an endogenous gene via a single transgene in mice
Drawbacks of conditional gene deletion in mice include the need for extensive breeding and, often, a lack of cell type-specificity. CRISPR interference (CRISPRi) is an alternative approach for loss-of-function studies that inhibits expression by guiding a transcriptional repressor to the transcripti...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6872636/ https://ncbi.nlm.nih.gov/pubmed/31754144 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-53611-6 |
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