A strategy to identify protein-N-myristoylation-dependent phosphorylation reactions of cellular proteins by using Phos-tag SDS-PAGE

To establish a strategy for identifying protein-N-myristoylation-dependent phosphorylation of cellular proteins, Phos-tag SDS-PAGE was performed on wild-type (WT) and nonmyristoylated mutant (G2A-mutant) FMNL2 and FMNL3, phosphorylated N-myristoylated model proteins expressed in HEK293 cells. The di...

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Enregistré dans:
Détails bibliographiques
Publié dans:PLoS One
Auteurs principaux: Kinoshita-Kikuta, Emiko, Tanikawa, Ayane, Hosokawa, Takuro, Kiwado, Aya, Moriya, Koko, Kinoshita, Eiji, Koike, Tohru, Utsumi, Toshihiko
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2019
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6872159/
https://ncbi.nlm.nih.gov/pubmed/31751425
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0225510
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