Integrative analysis of pooled CRISPR genetic screens using MAGeCKFlute

Genome-wide screening using CRISPR coupled with nuclease Cas9 (CRISPR/Cas9) is a powerful technology for the systematic evaluation of gene function. Statistically principled analysis is needed for the accurate identification of gene hits and associated pathways. Here, we describe how to perform comp...

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發表在:Nat Protoc
Main Authors: Wang, Binbin, Wang, Mei, Zhang, Wubing, Xiao, Tengfei, Chen, Chen-Hao, Wu, Alexander, Wu, Feizhen, Traugh, Nicole, Wang, Xiaoqing, Li, Ziyi, Mei, Shenglin, Cui, Yingbo, Shi, Sailing, Lipp, Jesse Jonathan, Hinterndorfer, Matthias, Zuber, Johannes, Brown, Myles, Li, Wei, Liu, X. Shirley
格式: Artigo
語言:Inglês
出版: 2019
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Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC6862721/
https://ncbi.nlm.nih.gov/pubmed/30710114
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-018-0113-7
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